However, unlike the RNAseq results, no significant difference was detected for the up-regulated genes, PROX1, SEMA3B (Figures 5G–J; Supplementary Figure S4), and the down-regulated gene, OASL (Figures 6I, J; Supplementary Figure S4), when comparing HbSC patients affected or unaffected by retinopathy by qRT-PCR. This evidence concerns the gene PROX1 and retinal disorder.