Due to the crucial role of EMT in CRC metastasis, we detected the effects of ZG16 on EMT represent markers by western blot, which showed that overexpression of ZG16 in HCT116 and SW480 cells led to elevated levels of E-cadherin, a marker of epithelial cells, and reduced levels of N-cadherin, a marker of mesenchymal cells (Figure 5B). The gene discussed is ZG16; the disease is colorectal carcinoma.