Overall, the results of this study indicated that PM2.5 exposure inhibited the transepithelial anion Isc by downregulating P2Y2R/CFTR pathway and P2Y2/calcium-dependent chloride channel, and this process may participate in aggravating the airway hyperresponsiveness, airway inflammatory, collagen deposition and hyperplasia of goblet cells. This evidence concerns the gene P2RY2 and airway hyperresponsiveness.