This study identified mutations in exons 7 and 8 of the PTEN gene in 2.2% of CRC cases, along with loss of PTEN expression in 34.9% of CRC cases; notably, loss of PTEN expression exhibited heterogeneity across different anatomical locations: proximal colon cancer was associated with advanced Dukes stage and lower differentiation level, while distal colon cancer showed an association with earlier Dukes stage. Here, PTEN is linked to colorectal carcinoma.