Larger opening volumes correlated with increased levels of AD biomarkers, including Aβ42 (R2 = 0.74), Tau (R2 = 0.95), and P-Tau181 (R2 = 0.86), assayed in serum-derived EVs sampled 3 days after FUS (N = 5). This evidence concerns the gene MAPT and Alzheimer disease.