In this part experiments, we preconditioned the mice with Oxamate (a lactate dehydrogenase A [LDHA] inhibitor) via intraperitoneal injection and followed by the construction of middle cerebral artery occlusion (MCAO) model, then mainly observed the brain injury at two stages of ischemia and reperfusion, respectively, following the workflow (Figure 1A). Here, LDHA is linked to ischemia.