We further collected BLCA pathological specimens and para-cancer tissue for immunohistochemical staining of OTUB1 and β-catenin in our hospital, the results showed that the expression of OTUB1 and β-catenin was consistent (Fig. S2B-D), OTUB1 was closely related to β-catenin (Fig. S2E). Here, OTUB1 is linked to bladder transitional cell carcinoma.