Moreover, immunofluorescence (IF) assays confirmed that the expression of XBP1 in the liver tissues of fat-, fructose- and cholesterol-rich (FFC) diet-fed 24-week-MASH mice was higher than in CD-fed mice (Fig. S1B), suggesting that XBP1s may play an essential role in the process of MASH progression. The gene discussed is XBP1; the disease is metabolic dysfunction-associated steatohepatitis.