Though leflunomide treatment had no obvious effects on the indicators (IL-1β, TNFα, and IL-6) of systemic inflammation in the plasma of the ApoE-/- mice compared to that of the WD mice (Figure 1F), it decreased the mRNA expression levels of Il-1β and Tnfα in peritoneal macrophages (Supplementary Figure 1A-B). This evidence concerns the gene TNF and Wilson disease.