However, western blotting analysis showed a significant increase in the phosphorylation level of PKCα in breast cancer platelets compared to its level in normal platelets, and immunofluorescence staining revealed the translocation of PKCα from the cytoplasm to the cell membrane, indicating the activation of PKCα in breast cancer platelets (Fig. 4B, 4C). Here, PRKCA is linked to breast carcinoma.