NFKB1 and infection: EVs from THP‐1 cells infected with trypomastigotes were studied. EVs interacted with TLR2 and increased the percentage of infected cells. EVs from T. cruzi‐infected macrophages induced NF‐κB translocation, altering gene expression of proinflammatory cytokines and STAT‐1/STAT‐3 signalling pathways. EVs induce the host inflammatory response during infection.