An overview showing the utilization of unconventional molecular profiling techniques, such as Single‐cell RNA sequencing (scRNA‐seq) and Visium In situ Capturing, employed to unravel the modulatory role of NK2 homeobox 1 (NKX2‐1) within the immune microenvironment of lung adenocarcinoma (LUAD). Here, NKX2-1 is linked to lung adenocarcinoma.