Together, 47 known and 9 previously unknown (Extended Data Fig. 3e) mutational signatures were identified, of which SBS28 and DBS-CRC5 were associated with POLE mutant MSS CRC, the SBS-CRC1, DBS-CRC3 and ID-CRC1 signatures with MMR, and the DNA MMR SBS44 signature in HM tumours with longer OS. This evidence concerns the gene POLE and neoplasm.