NSUN2 and infection: Further, we used the SARS-CoV-2 GFP/ΔN trVLP with NSUN2-mediated m5C methylation (normal m5C) and SARS-CoV-2 GFP/ΔN trVLP without NSUN2-mediated m5C methylation (low m5C) to infect Caco-2–N-mCherry cells and NSUN2 knockout Caco-2–N-mCherry cells at the same multiplicity of infection (MOI) (Fig. 5A).