AIF1 and Stroke: In addition, the overlap between neurons and microglia could not be an experimental artifact caused by non-specific immuno-staining of neurons or the expression of the microglial marker Iba1 by neurons, because ablation of microglia by PLX3397 prior to stroke completely removed any sign of overlap between neurons and microglia (n = 6–9 mice per group; P = 0.4281, unpaired t test) (Fig 2G).