In the mouse model of stoke, compared to PBS treatment, cRGD treatment (1 mM x 5 μl, i.c.v. into the contralateral hemisphere) immediately prior to ischemic onset had no effect on the interaction between neurons (NeuN+) and microglia (Iba1+) (n = 6–7 mice per group; P = 0.7686, unpaired t test) (Fig 3D and 3E) or the density of microglia (P = 0.4122, unpaired t test) (Fig 3F) in mice subjected to stroke induced by dMCAo. This evidence concerns the gene AIF1 and stroke disorder.