Additionally, portal hypertension is also related to HSCs contraction, which is mainly due to factors such as endothelin and catecholamines acting on G protein-coupled receptors (GPCRs) or the RhoA/Rho kinase pathway, inhibiting myosin light chain phosphatase (MLCP) to further constrict HSCs, thus leading to hepatic sinusoidal stenosis and inducing portal hypertension (Iwakiri and Trebicka, 2021). Here, RHOA is linked to portal hypertension.