This article performed a comprehensive study on the expression pattern and prognostic relevance of HIGD1B in gastric cancer using bioinformatics analysis, elucidated its potential involvement in critical pathways, explored the effects of HIGD1B on the tumor microenvironment (TME) and tumor-infiltrating immune cells (TIICs), and projected the immuno- and chemotherapeutic effects of GC based on HIGD1B expression. This evidence concerns the gene HIGD1B and neoplasm.