In CRC, with the combined application of multiplexed ion beam imaging by time of flight (MIBI-TOF) and antibody-based single-cell metabolic regulatory profiling (scMEP), Hartmann et al. uncovered a CD8+ T cell subset with CD39 and PD-1 expression that was metabolically repressed and excluded from the tumor-immune boundary, indicating niche-driven modulation of immune cell distribution and functionality [278]. The gene discussed is CD8A; the disease is colorectal carcinoma.