To assess the effect of TH analogs in vivo, T3, GC-1, and ZTA-261, either at low (0.1 μmol/kg day) or high (1 μmol/kg day) doses, were injected intraperitoneally into the high-fat diet (HFD)-induced obesity model mice according to the previous report28. Here, TH is linked to obesity due to melanocortin 4 receptor deficiency.