To investigate the potential role of CYP4F3 on CRC cell proliferation and migration, we established a stable overexpression of CYP4F3 in the CT26.wt and SW620 cell line, denoted as CT26.wt-OE and SW620-OE, along with its corresponding control, CT26.wt-NC and SW620-NC, which was established using empty vector construct. Here, CYP4F3 is linked to colorectal carcinoma.