We introduced CD8α-EGFP (23) and a TCRα/β specific to a NY-ESO-1 peptide antigen-loaded MHC class I (14) with CD3ε and CD3ζ (24) into Jurkat T cells via sequential infections with recombinant lentivirus (25) (SI Appendix, Fig. S12A). Here, CD3E is linked to infection.