Both everolimus (0.33 ± 0.06 versus 0.57 ± 0.04, P = 0.002) and rapamycin (0.28 ± 0.07 versus 0.57 ± 0.04, P = 0.002) reduced the phosphorylation ratio of NKCC1 pThr203,207,212 compared with total NKCC1, whereas BYL719 did not modify the dephosphorylation of NKCC1 pThr203,207,212 (0.55 ± 0.11 versus 0.57 ± 0.04) relative to total NKCC1 (P = 1) (Fig. 4B) with n = 4 western blots and N = 4 MCD patients (two FCD, one TSC and one HMG). The gene discussed is SLC12A2; the disease is tuberous sclerosis.