Peripheral blood mononuclear cells (PBMCs) from a healthy adult subject were cultured with M-CSF and GM-CSF for 5 days, followed by co-infection with a combination of pLVCD66b−scFv−cherry and pLVhTNF lentiviruses to generate adoptive DCs expressing scFv on their surface and secreting TNFα, which we called Muc1-directed DCs producing TNFα (M-DCsTNF). This evidence concerns the gene CSF1 and coinfection.