We previously performed spatial proteomic profiling on overlapping samples from the RCC TMAs to characterize expression patterns of multiple tumor immunomarkers, including CD3, PD-1, PD-L1, LAG3, TIM-3, and CTLA-4 in immune cells (CD45+), and PD-L1 in tumor cells (CK+) [9, 10]. The gene discussed is CTLA4; the disease is renal cell carcinoma.