also spiked H1975 cells together with A549 and SK‐MES lung cancer cell lines (100 cells/5 mL) to compare semiautomated approaches ISET and Parsortix® with manual enrichment methods, namely density gradient medium and erythrocyte lysis buffer, along with their combined usage with CD45 depletion technology employing beads, Dynabeads [66]. The gene discussed is PTPRC; the disease is lung cancer.