To investigate the effect of RBM43 on HCC metastasis further, we constructed RBM43-deficient HepG2 and QGY-7703 cells through CRISPR-Cas9 technology and successfully reconstituted RBM43 in knockdown HCC cell lines by lentivirus infection (Fig. S2A–D). This evidence concerns the gene RBM43 and hepatocellular carcinoma.