At the single-cell level, only Golgi Ras activity (measured by Golgi Ras-LOCKR-S FRET ratios) correlated (R2 = 0.78, H358 cells) with Erk activation (pErk immunostaining) 72 h after AMG-510 treatment in the KRas-G12C-driven cancer cell lines that were tested (Extended Data Fig. 3f,g). This evidence concerns the gene KRAS and cancer.