To investigate the role of SMYD5 in HCC tumorigenesis from the cirrhosis stage, we interbred conditional Smyd5loxP/loxP mutant mice with hepatocyte-specific Cre-recombinase strain (AlbCre), resulting in specific Smyd5 gene deletion in the liver (Fig. 6e). Here, SMYD5 is linked to hepatocellular carcinoma.