MYCN-amplified tumors are generally considered “cold,” with low immune cell infiltration, while the non-MYCN-amplified are regarded as “hot,” containing several types of immune cells (B, NK, NKT cells, M2 macrophages).74 Single-cell RNA-sequencing technologies defined the NB immune landscape, with 27 different immune subtypes, dysfunctional T cells, and accumulation of immunosuppressive cells.15,16 The immune system can be exploited for therapy, as described in the Immunotherapy section. Here, MYCN is linked to neuroblastoma.