This has also been confirmed by recent study that in HFD-induced NAFLD and an FFAs-induced cellular models, targeted knockdown of Notch-1 regulates the PI3K/NLRP3 pathway through SHP2 phosphorylation, thereby mitigating NAFLD (Gao et al., 2023). This evidence concerns the gene PTPN11 and metabolic dysfunction-associated steatotic liver disease.