To further validate whether SAC3D1 has an impact on the progression of hepatocellular carcinoma (HCC) in vivo, we conducted subcutaneous tumorigenesis experiments by injecting HCCLM3 cells with knocked-down SAC3D1 and control HCCLM3 cells into the subcutaneous tissue of nude mice on the same side of the armpit. The gene discussed is SAC3D1; the disease is hepatocellular carcinoma.