Previous studies have shown that human LYZ inhibits LPS‐induced inflammation in human endothelial cell and mouse sepsis by regulating the expression of HMGB1.[15] More importantly, HMGB1 is associated with mastitis disease, and binds to receptors such as TLR2 and TLR4 to enhance the inflammatory response.[23] However, whether dairy goat LYZ exerts its anti‐inflammatory effects by regulating the expression level of HMGB1 is unknown, which we verified. This evidence concerns the gene TLR4 and Sepsis.