Steady cell lines using OTUD3 downregulation and upregulation lentivirus were developed in three different DLBCL cell lines, namely Farage, OCI-LY-1, and Su-DHL-4 to examine the role of OTUD3 in DLBCL cells, and the efficiency of genetic manipulation was verified using Western Blotting (WB) (Figs. 1B, S1F). This evidence concerns the gene OTUD3 and diffuse large B-cell lymphoma.