Interestingly, we could detect an aging-dependent appearance of ALS-related phenotypes as MNs were cultured longer: 5-weeks-old SYT13+/− cultures showed an aberrant accumulation of SQSTM1/p62 aggregates (Fig. 2D) and of cytotoxic, perinuclear aggresomes (Fig. 2E), without displaying any sign of pathological cytoplasmic accumulation of TDP43 (Fig. 2F). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.