In KEGG enrichment, 75 pathways were enriched by overlapping differential genes.The results show the top 10 pathways in p.adjust, including Prolactin signaling pathway, Pathogenic Escherichia coli infection, JAK − STAT signaling pathway, Cytokine − cytokine receptor interaction, Yersinia infection, PD − L1 expression and PD − 1 checkpoint pathway in cancer, Neurotrophin signaling pathway, Insulin resistance, C − type lectin receptor signaling pathway, Adipocytokine signaling pathway (Fig. 3D). The gene discussed is CLEC4D; the disease is escherichia coli infection.