To further explore antigen heterogeneity in pediatric AML, 10 primary AML samples (mean percentage of blasts 85% (76–92%), mean number of analyzed AML blasts per patient: 75.700 (28.000–143.000)) were analyzed by dimensionality reduction using uniform manifold approximation and projection (UMAP) based on the expression of CD45, CD3, CD34, CD33, CD38, CD123, CD135 and CD371 as well as FSC and SSC signals. The gene discussed is FLT3; the disease is acute myeloid leukemia.