Previous studies have reported elevated NLRP3 levels in patients with varicocele or azoospermia, indicating its potential role in male infertility.[27] It has been found that NLRP3 promotes obesity-related spermatogenesis impairment and is involved in the production of inflammatory factors that can disrupt normal spermatogenic function.[28] Additionally, Poli et al[29] reported that the production of nitric oxide and inflammatory factors can induce the assembly of the NLRP3 inflammasome complex through the differentiation of testis macrophages. Here, NLRP3 is linked to Azoospermia.