The survival analysis suggested that a higher number of tumor cells around CD4+Tregs (IM, 51.33 vs. no reached, P = 0.020; TC, 39.93 vs. not reached, P < 0.001) and CD8+Tregs (IM, 46.93 vs. not reached, P = 0.002; TC, 45.03 vs. not reached, P < 0.001) showed a lower RFS both in IM and TC (Fig. 3f–i). This evidence concerns the gene CD8A and neoplasm.