HLA-DRB5, a gene previously identified as downregulated in cancer tissues compared to adjacent normal tissues in our bulk RNA sequencing analysis, exhibited a decrease in expression as Type II Alveolar Epithelial Cells transitioned from cluster 0 to cluster 1 and as Microvascular Endothelial Cells progressed from cluster 3 to cluster 4. The gene discussed is HLA-DRB5; the disease is cancer.