At the 48 h infection time point, Hsp22, Hsp100, Hsp27, and DIS3 were confirmed to be significantly downregulated (∗p ≤ 0.05), significantly upregulated (∗p ≤ 0.05), slightly downregulated, and significantly upregulated (∗p ≤ 0.05), respectively in FCoV-derived EVs in contrast with the uninfected control EVs. This evidence concerns the gene DIS3 and infection.