SLC6A3 and Parkinson disease: To investigate the effect of GDNF on DAT in DA neurons, we evaluated the expression and localization of DAT in PD cells and mice using western blot and immunofluorescence techniques.In cell experiments, different concentrations of GDNF (0 ng/mL, 25 ng/mL, 50 ng/mL, 100 ng/mL, 200 ng/mL) and MPP were used Co-intervention of MES23.5 cells for 24 h, and then cell membrane cytoplasmic proteins were extracted to detect the expression of glycated DAT (Gly-DAT, 80 kDa) and non-glycated DAT (Nongly-DAT, 55 kDa).