First, we infected WT and CD1d-KO mice with DENV2 (2 × 105 PFU) via s.c. injection and determined the relative NKT cell subtypes present in the FP skin and dLN by flow cytometry at days 3 and 5 after infection (Figure 2A), which were defined based on cellular markers identified in prior reports (13, 15, 39, 40). This evidence concerns the gene CD1D and infection.