SH-SY5Y and SK-N-MC cells have been widely used in neuroblastoma research16,17and our previous study has shown NVP-BEZ235 (BEZ235), a dual PI3K and mTOR inhibitor18 declined the expression of PI3K/mTOR pathway-related proteins in both cells.19 Therefore, both cells were used to evaluate the roles and mechanism of autophagic degradation of the CDK4 on cell cycle distribution. Here, MTOR is linked to neuroblastoma.