In summary, our findings provide novel evidence that ozenoxacin effectively inhibits sebum production and accumulation by suppressing the expression of DGAT‐1, SCD‐1, and perilipin‐1 by inhibiting the mTORC1 signaling pathway in differentiated sebocytes, therefore ozenoxacin is a promising treatment for acne therapy by inhibiting microbial proliferation and effectively suppressing sebum production. The gene discussed is DGAT1; the disease is acne.