In the present study, treatment of anemia in animals using PVPE demonstrated positive effects through biochemical and cellular pathways, including decreasing the serum markers such as ALT, AST, ALP, Bili T, and Bili D. It is well documented that herbs with antioxidant effects mitigate the PHZ-induced hepatotoxicity via modulating liver enzymes (Henneh et al., 2020). This evidence concerns the gene GPT and anemia (phenotype).