Furthermore, a recent study associated TMB with response to adjuvant anti-PD-1 treatment in 165 melanoma patients; in this study, samples were sequenced using a multigene next-generation sequencing (NGS) panel to identify the mutational load before treatment onset, and it was shown that patients with higher TMB values experienced better outcomes and extended relapse-free survival (RFS). The gene discussed is PDCD1; the disease is melanoma.