Since altered DNA methylation has been identified as a key determinant of MASLD pathogenesis (Li et al., 2018; Hyun and Jung, 2020; Lai et al., 2020; Bu et al., 2022; Kuramoto et al., 2022; Melton et al., 2023), we next applied Infinium mouse methylation Beadchip array studies to map genome-wide DNA methylation changes in liver biopsies of WT and hepatocyte-specific PPARα KO mice, following 6-week chow or CDAHFD diet. This evidence concerns the gene PPARA and metabolic dysfunction-associated steatotic liver disease.