Finally, cortical organoid-derived astrocytes from C9orf72-ALS patient iPSCs as well as sALS iPSC-derived astrocytes showed perturbed autophagy and P62 accumulations (Figure 3) (Szebényi et al., 2021; Baofengfeng et al., 2022), which argues for a role of autophagy across multiple cell types. This evidence concerns the gene C9orf72 and amyotrophic lateral sclerosis.