In this study, we generated CAR T cells with cytoplasmic domain combinations of 4-1BB instead of CD28 that can target both human FAP (hFAP) and mouse FAP (mFAP), and detected the killing function of FAP-CAR T on FAP+ tumor cells as well as human and murine primary CAFs in vitro and in vivo. The gene discussed is FAP; the disease is neoplasm.