Indeed, THP-1 and BMDMs-derived macrophages treated with conditioned medium (CM) from human or mouse GBM cells with TNFRSF14 knockdown showed a significantly decreased chemotaxis (Fig. 3E and Figure S3D, F) and anti-inflammatory polarization capabilities, compared to CM from control group (Fig. 3F, G and Figure S3F-J). This evidence concerns the gene TNFRSF14 and glioblastoma.