qPCR analysis also revealed that mRNA of pro-inflammatory markers (CD80, iNOS and IL-6) significant increased while anti-inflammatory markers (CD163 and CD206) decreased in both of THP1- and BMDMs-derived macrophages with treatment of CM from TNFRSF14 knockdown human or mouse GBM cells (Fig. 3F and Figure S3G). The gene discussed is CD80; the disease is glioblastoma.