Then, we employed IFN-α, IFN-β, and IFN-γ (0, 20, 50, 100 ng/ml) to incubate two GBM cell lines (one primary GBM cell line, GSC1, and one conventional GBM cell line, U87) to induce IFN signaling activation in GBM cells in vitro. This evidence concerns the gene IFNA1 and glioblastoma.